Tools for interpreting FRET data
ExiFRET is a web based tool to determine the FRET efficiency for complex arrangements of fluoropores including those with multiple donors and acceptors.
thetaFRET is a web based tool to help understand FRET when fluorophores have constrained orientations.
publication describing exifret and thetafret
HandyFRET is a packet of scripts making use of simple methods to account for dye diffusion and orientation during FRET data interpretation.
Tools for measuring FRET efficiency
FRETTY
is an ImageJ plugin for determining FRET
efficiencies using spectral unmixing of both
excitation and emisison spectra. This can be
used to directly account for direct excitation
of acceptor fluorophores and bleed through of
donor emission into the acceptor channel.
The actual Plugin (jar format) Bugs fixed 7 July 2014
User guide (pdf format)
example video basic use. (avi format) Smaller version
example video with spectral correction. Smaller version
example images to allow you to try it out
open access publication describing the method
The Jama package needed for newer versions of ImageJ
FRET_ROI.java is an ImageJ plugin for calculating FRET for a ROI using acceptor photobleaching.
FRETcalc_ben.java Plugin for pixel by pixel FRET analysis using acceptor photobleaching. Minor variation on the program of David Stepensky
Tools for analysis of NMR data
NMR 2D analysis
ImageJ plugin for opening multiple
experiments, analysing and integrating peaks in
2D NMR spectra.
User guide
for NMR 2D analysis
Bruker opener
files in case required - see user guide
publication describing the method. supp info