Welcome to ExiFRET
ExiFRET is a simple and flexible program that calculates FRET efficiencies between fluorophores in complex geometries, to help design and understand FRET experiments. This page allows you to run the program ExiFRET over the web and retrieve the output files for your own use. A short background to the program, as well as a more detailed description of the input parameters, examples of how to use the program and publications describing the method can be found by following the tabs above.
Example input files for the Figures found in the newest ExiFRET publication can be found in examples 2 .
To run the program simply enter the required input parameters in the form below and click on the "Run ExiFRET" button. The output of the program will be displayed below. You can also download the calculated FRET efficiencies as a text file.
For cases where fluorophores have constrained orientations, please use thetaFRET.
ExiFRET is developed in the Corry Research Group at the Australian National University.